OVERVIEW
Blasticidin S is a peptidyl nucleoside antibiotic isolated from the culture broth of Streptomyces griseochromogenes. It specifically inhibits protein synthesis in both prokaryotes and eukaryotes through inhibition of peptide-bond formation in the ribosomal machinery.
Blasticidin S is used to select transfected cells carrying bsr or BSD resistance genes (1-4).
References
- IZUMI M., MIYAZAWA H., KAMAKURA T.,YAMAGUCHI I., ENDO T.and F.HANAOKA. 1991. Blasticidin S-resistance gene (bsr): a novel selectable marker for mammalian cells.Exp.Cell Res.197: 229-233
- ITAYA M.,YAMAGUCHI I., KOBAYASHI K., ENDO T.and T.TANAKA. 1990. The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. J.Biochem. 107: 799-801
- KIMURA M., KAMAKURA T.,TAO Q.Z., KANEKO I and I.YAMAGUCHI. 1994. Cloning of the blasticidin S deaminase gene (BSD) from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae. Mol. Gen. Genet. 242: 121-129
- KIMURA M.,TAKATSUKI A and I.YAMAGUCHI. 1994. Blasticidin S deaminase gene from Aspergillus terreus (BSD) a new drug resistance gene for transfection of mammalian cells. Biochim.Biophys.Acta.1219:653-659
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